April 21st, 2019 9:49 PM by Dan Howard
There are several testing methods used for diagnosing mold issues. As in all things in the world, there are advantages and disadvantage to each type of testing. That means that each type of testing is useful in its own way….and often not appropriate for other applications.
ERMI (Environmental Relative Moldiness Index) is one test that is not often used or understood. ERMI is the product of the modern miracle of DNA technology. The EPA owns the patent on the process and limits its use to approved labs. The EPA also states that their approval of the technology is only “experimental.”
However, there are many studies and anecdotal evidence of the benefit of the test results for patients with CIRS (Chronic Inflammatory Respiratory Disease) There are also some very interesting, but limited studies that high ERMI scores correlate with high lactate in the brain. High lactate correlates with cognitive problems. It may be that identifying high ERMI score conditions may be useful in treating some diseases. These are still very preliminary studies and require more research.
Let’s do the Pros and Cons before we talk about how this technology works.
ERMI can give very targeted specific specialization for target molds that can have an influence on health
ERMI can give evidence of the historic (new or old mold contaminations) mold conditions in a building.
Historic evidence of long term exposure vs short term exposure can be useful for medical practitioners
ERMI does not quantify mold, it only identifies the 36 species of targeted molds
ERMI Originally specified old carpet that was not regularly cleaned to provide the source of dust to give that historic record. Now any dust from the home is acceptable. The age of dust is hard to know.
ERMI is still an evolving science in terms of correlation of ERMI results and health implications
ERMI does not help to isolate the source of the mold contamination to aid in any required remediation
Overview of the process
A sample of dust is taken using a specialized dirt trap. The dirt/dust/debris is to be collected by using a vacuum cleaner hooked up to a specialized air filter. An alternative system is a smaller cassette and a standard air sampling pump. The sample is supposed to be drawn from a roughly 2 square yard carpet area in either a living room or bedroom.
The sample is sent to an EPA licensed lab. The lab takes the dust from the dirt trap and puts it through a filter to isolate the small, mold size particles. (Think spaghetti in a strainer, only microscopic in size)
Those particles are put into a tube with a known amount of Geotrichum candidum
and the DNA is beat out of the mold spores with microscopic beads called “bead pellets.” That mush is then filtered and the sifted genetic stuff is mixed with a buffer solution. It is then dumped into a solution called “Master Mix” and put through a series of temperature controlled reactions.
If you are wondering the technical name for the magic chemistry we are doing, it is MSQPCR
Mold Specific Quantitative Polymerase Chain Reaction
Now remember that known quantity of Geotrichum candidum that was in the mix? That is the reference basis that can be used to compare the assays (checking process) for each of the target molds (molds that they are looking for). The checking process is done with a “Sequence Detector” (which is identifying DNA sequences)
Once the 36 target molds are identified, the 26 in the WDB (Water Damage Building) group are measured and are compared to the common or outdoor group of 10 molds.
The reason for the look at the ratio of the two is that the exact quantity of each mold is not determined by this test. That is the result of several factors. Our size of sample could be small or big, based upon the amount of dirt we swept up. We can’t figure out by counting pieces of DNA if there is a little or a lot of mold in the building. You get the point. We do not know how much mold is in the building from an ERMI test.
What we do know is that if most of the mold DNA is the outdoor molds, then there is less mold growing in the house. If there is a whole bunch more of the indoor mold than the exterior, oops, there is a lot more mold growing IN the house than coming in from the OUTSIDE.
Scoring is done on a scale of -10 to 20. The higher the number, the more mold that is from growing inside the house in the tested building area. That ERMI Score number is a “sort of number,” not an exact measure. It is based on a limited number of tests from a limited geographic area. Hence reference to it as a “Relative Score.”